Heys will be away to West Didsbury and Chorlton in the Lancashire Challenege Trophy 2nd Round.
Following their success at Padiham on Tuesday, Heys will travel to the NW Counties Division One South side in early November on a date to be confirmed.
The teams met in pre-season with Didsbury edging a tight contest 1-0. The game will also see Heys come up against former players Lee Grimshaw and Rob Sadler, the latter being part of the Coaching Staff there.
The Full draw is:
Colne vs Bacup Borough
Charnock Richard vs Lancaster City
Radcliffe vs Darwen
Nelson vs Bamber Bridge
West Didsbury & Chorlton vs Prestwich Heys
Wythenshawe Amateurs vs Daisy Hill
Clitheroe vs Atherton Collieries
Southport vs Ashton Athletic